中国中西医结合杂志2012，Vol.32Issue(9)：1275-1279,5.
经前平颗粒对经前期综合征肝气逆证大鼠脑μ阿片受体mRNA及蛋白表达的影响
Effects of Jingqianping Granule on mRNA and Protein Expression of μ Opioid Receptor in Premenstrual Syndrome Gan-qi Invasion Rats
摘要
Abstract
Objective To observe the effects of Jingqianping Granule (JG) on mRNA and protein expres-sions of m opioid receptor in the parietal cortex and the frontal cortex, the hypothalamus and hippocampus of pre-menstrual syndrome (PMS) Gan-qi invasion rats. Methods Twenty rats were selected to prepare the PMS Gan-qi invasion model. After modeling rats were divided into the model group and the Chinese herb treated group, ten in each group. Another 10 rats were selected as the normal control group. During the modeling, JG (1.6 g/kg) was given to rats in the Chinese herb treated group by gastrogavage, while equal volume of normal saline (1 mL/100 g) was given to rats in normal control group and the model group. All treatment was performed once daily for five suc-cessive days. The mRNA and protein expressions of m opioid receptor in the parietal cortex and the frontal cortex, the hypothalamus and hippocampus were detected using RT-PCR and Western blot respectively. Results Com-pared with the normal control group, the bands of products of MOR mRNA and protein in the parietal cortex and the frontal cortex were relatively weaker in the model group, and the optical density value decreased. The MOR mRNA and protein expressions in the parietal cortex and the frontal cortex relatively decreased. But the bands of products of MOR mRNA and protein in the hypothalamus and hippocampus were relatively stronger and optic value in-creased. The MOR mRNA and protein expressions in the hypothalamus and hippocampus relatively increased with statistical difference (P<0. 01, P<0. 05). Compared with the model group, the bands of products of MOR mRNA and protein in the parietal cortex and the frontal cortex were relatively enhanced, the MOR mRNA expression in the parietal cortex increased, the MOR protein expression in the parietal cortex and the frontal cortex increased in the Chinese herb treated group. The bands of products of MOR mRNA and protein in the hypothalamus and hippocam-pus were relatively weaker. The MOR mRNA and protein expressions in the hypothalamus and hippocampus rela-tively decreased. The MOR protein expression in the hippocampus decreased relatively with statistical difference (P <0, 01, P <0. 05). Conclusions Expression of μ opioid receptor in brains of PMS Gan-qi invasion rats was re-gionally specific. Administration of JG showed corresponding regulatory effects.关键词
经前期综合征肝气逆证/μ阿片受体/不同脑区/经前平颗粒Key words
premenstrual syndrome Gan-qi invasion/ μ opioid receptor/ different brain regions/ Jingqian-ping Granule引用本文复制引用
王芙蓉,薛玲,张惠云..经前平颗粒对经前期综合征肝气逆证大鼠脑μ阿片受体mRNA及蛋白表达的影响[J].中国中西医结合杂志,2012,32(9):1275-1279,5.基金项目
国家自然科学基金重点项目(No.30930110) （No.30930110）
国家自然科学基金面上项目(No.30973688,No.81173162) （No.30973688,No.81173162）
