中药材2012，Vol.35Issue(3)：443-446,4.
白藜芦醇对Hepa1-6肝癌细胞凋亡和ROS的影响
Effects of Resveratrol on Apoptosis and ROS Production in Hepa 1-6 Hepatocarcinoma Cells
摘要
Abstract
Objective:To observe the effects of resveratrol on apoptosis and ROS production in murine hepatocarcinoma Hepa 1-6 cells in vitro. Methods: Hepa 1-6 cells were treated with different dose of resveratrol(20 μmol/L,40 μmol/L,80 μmol/L). Cell proliferation was detected with MTT assay at 24 h,48 h and 72 h. Hoechst 33258 staining was used to visualize apoptotic morphology. Cell apoptosis was detected by flow cytomctry analysis. Activated caspase-3 was detected by western blot. Intracellular ROS production was observed by 2',7'-dichlorofluorescin diacetate( DCF-DA) staining. Results:Compared with the control, upon treatment with 20-80 μmol/L resveratrol for 24 h,48 h or 72 h,the proliferation of Hepa 1-6 cells was significantly inhibited in a time-dose dependent manner. 20-80 |unol/L resveratrol also induced apoptosis and apoptotic morphology change in Hepa 1-6 cells accompanied with caspase-3 activation and ROS generation. Conclusion: Resveratrol could inhibit cell proliferation, induce apoptosis in murine hepatocarcinoma Hepal-6 eells,and the mechanism may associated with caspase-3 activation and ROS production.关键词
白藜芦醇/肝癌/细胞凋亡/Caspase-3/活性氧Key words
Resveratrol/Hepatocarcinoma/ Apoptosis/Caspases-3/Reactive oxygen species( ROS)分类
医药卫生引用本文复制引用
杜琴,沈克平,胡兵,邓珊..白藜芦醇对Hepa1-6肝癌细胞凋亡和ROS的影响[J].中药材,2012,35(3):443-446,4.基金项目
上海市基础研究重点项目(09JC1413600) （09JC1413600）
