中药材2012，Vol.35Issue(3)：467-470,4.
牛蒡苷元制备方法的研究
Research on the Preparative Method of Arctigenin
摘要
Abstract
Objective: To research on the preparation of Aretigenin in vitro. Methods: Took enzyme concentration,time course and substrate concentration as investigation factors,used Box-Behnken design-response surface methodology to optimize the enzyme hydrolysis path of Aretigenin. Results: The best operational path for Aretigenin was as follows; the temperature was 50 ℃ ,pH was 4. 8,enzyme concentration was 0.44 U/mL,time course was 46.81 min, substrate concentration was 0.29 mg/mL,the conversion rate was 90.94%. Conclusion: This research can be regarded as a reference in preparing Aretigenin in vitro.关键词
牛蒡苷/牛蒡苷元/β-葡萄糖苷酶Key words
Arctii/ Aretigenin/ β-Glucoside分类
医药卫生引用本文复制引用
张李赢,杨轶舜,张彤,丁越,蔡贞贞,陶建生..牛蒡苷元制备方法的研究[J].中药材,2012,35(3):467-470,4.基金项目
教育部新世纪人才计划(NCET-10-0944) （NCET-10-0944）
国家自然科学基金(81173561) （81173561）
上海市教委曙光计划(10SG40) （10SG40）
上海市重点学科(J50302) （J50302）
