中药材2012，Vol.35Issue(3)：475-479,5.
天花粉凝集素的提取工艺及降糖活性研究
Study on the Extraction Technology and Hypoglycemic Activity of Lectin from Trichosanthes kirilowi
摘要
Abstract
Objective: To extract lectins from Trichosanthes kirilowi and study their hypoglycemic activity. Methods: The optimal extraction process included the following parameters were conformed by optimization analysis,lectins extracted from Trichosanthes kirilowi was Achieved by ammonium sulfate precipitation;The agglutinate activity was determined by using the agglutination test with 5% human blood cells. Human hepatocarcinoma cell HepG2 and the alloxan-induced diabetic mice model were used to assess hypoglycemic activity of Lectin in Trichosanthes kirilowi. Results: The agglutination indexes of lectins extraction buffer were 32;The cell and mice tests indicated that the lectins exhibited hypoglycemic activity in the 70% saturation. Conclusion: The optimum extraction technology is as follows: extraction with PBS,the material-water ratio is 1:30,the extraction lime is 24h,while the concentration of sodium chloride is 0 mol/L and pH is 7. 2. Precipitate lectins by ammonium sulfate in the 70% saturation,centrifugal speed is 10 000 r/min. Lectins extracted from Trichosanthes kirilowi exposes proper hypoglycemic activity.关键词
天花粉/凝集素/提取工艺/降糖活性Key words
Trichosanthes kirilowi Maxim. /Lectins/Extraction technology/Hypoglycemic activity分类
医药卫生引用本文复制引用
李琼,叶小利,曾红,陈新,李学刚..天花粉凝集素的提取工艺及降糖活性研究[J].中药材,2012,35(3):475-479,5.基金项目
中央高校基本科研业务费(XDJK2011C051) （XDJK2011C051）
