中药材2012，Vol.35Issue(5)：749-756,8.
红芪多糖对S180瘤细胞化疗协同增效作用的蛋白质组学分析
Proteomics Analysis of the Synergy Effect of Radix Hedysari Polysaccharides Combined with Chemotherapy on S180 Tumor Cells
摘要
Abstract
Objective: To analyze the differentially expressed proteins of the synergy effect of Radix Hedysari polysaccharides (HPS) combined with chemotherapy(Cy)on S180 tumor cells. Methods: The total proteins extracted from the HPS combined with Cy treated S180 cells in tumor-bearing mice were separated by two dimentional gel electrophoresis(2-DE)and compared with those from Cy treated S180cells using PDQuest 8. 0 software. Mass spectrometry was applied to identify the differentially expressed proteins. Western blot was used to determine the differential expression of one protein. Results:Twenty-four differentially-expressed proteins in HPS group were discovered. The five differential expressed proteins among twenty-four proteins were later identified by mass spectrometry and Mascot software as heat-shock protein hsp84( HSP90β),apolipoprotein A,albumin,heat shock protein beta-1 ( HSP27)and unnamed protein product,including one up regulated and four down regulated expressed proteins respectively. Results from Western blot manifested the same trend as from proteomics analysis. Conclusion: Proteomics technique can be used to discover target proteins associated with the synergy effect of HPS combined with Cy on S180 tumor cells,involving some important proteins related to energy metabolism,oxidative stress and apoptosis induction signal transduction.关键词
红芪多糖/S180瘤细胞/蛋白质组学/增殖抑制相关蛋白/凋亡诱导相关蛋白Key words
Radix Hedysari polysaccharides/S180 tumor cells/Proteomics/Proliferation inhibition-associated protein/Apoptosis induction-associated protein分类
医药卫生引用本文复制引用
卫东锋,赵春燕,程卫东,韦艳霞,张李峰,苏刚..红芪多糖对S180瘤细胞化疗协同增效作用的蛋白质组学分析[J].中药材,2012,35(5):749-756,8.基金项目
中国博士后科学基金特别资助(201003073) （201003073）
中国博士后科学基金资助(20090450017) （20090450017）
教育部高等学校博士点基金项目(200807330006) （200807330006）
