中医药学报2012，Vol.40Issue(2)：62-65,4.
Cocktail法评价注射用益气复脉(冻干)对大鼠肝微粒体CYP1A2、CYP3A的诱导作用
Effect of Yi Qi Fu Mai (YQFM) (Lyophilized) on the Activity of Hepatic CYP1 A2 and CYP3A in Rats
摘要
Abstract
Objective: To investigate the effect of Yi Qi Fu Mai Injection (YQFM) (Lyophilized) on the activity of cyto-chrome P450 (CYP1A2, CYP3A) in rats. Method: Healthy wistar rats raised ethically were pretreated with YOFM(Lo-phhilized) via the caudal vein once daily for consecutive seven days, and then hepatic microsomes were prepared. A cocktail selective substrates consisting of the phenacetin (PN,CYP1A2) and testosterone (TS,CYP3A) was incubated with rat livermicrosomes. Determined by HPLC method, the formation rates of acetaminophen and 6β-OH testosterone, the metabolites of the probe drugs were used as an indicator to estimate the activity of CYP1A2 and CYP3A. Result: The formation rates of acetaminophen were(18.04 ± 1.00) ,(43. 07 ±2.90) , (27. 6 ±4. 5) ng o ( mg protein)-1 min-1 ,The formation rates of 6β -OH testosterone were(15.79 ± 1.43 ) , (40. 86 ± 3. 32) , (32. 8 ± 3. 67 ) ng o ( mg protein) -1 o min-1. Conclusion; YQFM has an induced effect on the activity of CYP1A2 and CYP3A.关键词
注射用益气复脉(冻干)/Cocktail/CYP1A2/CYP3AKey words
Yi Qi Fu Mai Injection (Lyophilized) /Cocktail/CYP1A2/CYP3A分类
医药卫生引用本文复制引用
胡冰,岳洁皓,段超慧,叶正良,周大铮,李德坤,马英丽..Cocktail法评价注射用益气复脉(冻干)对大鼠肝微粒体CYP1A2、CYP3A的诱导作用[J].中医药学报,2012,40(2):62-65,4.基金项目
重大新药创制科技重大专项(2010ZX09602-004) （2010ZX09602-004）
重大创新专项资金(08FDZDSH01404) （08FDZDSH01404）
