中医药学报2012，Vol.40Issue(3)：24-28,5.
针药联合抑制缺血再灌注大鼠 PI3K/Akt信号通路的相关性研究
The study on Inhibitting Ischemia-reperfusion Rats'PI 3K/Akt Signal Pathways by Acupuncture Combining Medicine
摘要
Abstract
Objective: To explore the curative effect and mechanism of Xinnao Tongluo liquid preconditioning and elec-troacupuncture (EA) postconditioning with ischemic reperfusion of rats heart through apoptotic index, p - Akt. Methods; All rats were randomly divided in four groups: IR group, Xinnao Tongluo liquid preconditioning group, EA postconditioning group, Xinnao Tongluo liquid preconditioning and EA postconditioning group. Establishment of ischemia reperfusion injury model. Test the variety of apoptotic index p - Akt by immunol histochemistry , RT - PCR and western blot. Results: Xinnao Tongluo liquid preconditioning, EA postconditioning can decrease the infarct size and apoptotic index, increase p - Akt, Xinnao Tongluo liquid preconditioning and EA postconditioning group was better than other groups. Conclusion; 1. Xinnao Tongluo liquid preconditioning and EA postconditioning affect the contents of AktmRNA .2. Xinnao Tongluo liquid preconditioning and EA postconditioning can increase the expression of p - Akt, and it shows that PI3K/Akt pass way participate in the role of lightening ischemic reperfusion injury.关键词
心脑通络液预处理/针刺后处理/缺血再灌注/PI3K/Akt信号通路/凋亡指数Key words
Xinnao Tongluo liquid preconditioning/ Electroacupuncture postconditioning/ Ischemic reperfusion/ P13K/ Akt pass way/Apoptotic index分类
医药卫生引用本文复制引用
李丹,张志辰,李书霖,陈光..针药联合抑制缺血再灌注大鼠 PI3K/Akt信号通路的相关性研究[J].中医药学报,2012,40(3):24-28,5.基金项目
黑龙江省中医药管理局中医药科研项目(ZHY08-W51) （ZHY08-W51）
黑龙江省教育厅科研项目(11541337) （11541337）
