中医药信息2012，Vol.29Issue(4)：53-55,3.
补髓生血颗粒对慢性再障患者骨髓单个核细胞内Ca2+水平及不同血液疾病间差别的影响
Impact of Ca2+ Concentration in Bone Marrow Mononuclear Cells with Busuishengxue Granule in Chronic Aplastic Aaenia Patients and the Observation of It in Different Blood Diseases
摘要
Abstract
To observe the change of Ca + concentration in bone marrow mononuclear cells (BMMC) in chronic aplastic anemia (CAA) , exploring the possible mechanism of Busui Shengxue particles on CAA. To explore diagnosis and treatment ideas by observing the Ca2+ concentration in different blood diseases. Method; Extraction separation the CAA patients' BMMC. Use fluo3 AM staining by flow cytometry detection of intra-cellular calcium concentration. Detect the Ca + concentration in CAA, Myelodysplastic Syndrome(MDS) , Id-iopathic Thrombocytopenic Purpura(ITP) , Iron -Deficiency Anemia(IDA) and compare them. Results; CAA patients' BMMC intracellular Ca 2+ concentrations were significantly higher than those in the normal group. Af-ter Busui Shengxue particles treatment, patients in each group of intracellular Ca2+ concentration declined, Busui Shengxue particle group was better than the control group, but still higher than the normal group. MDS and CAA was higher than others. ITP, IDA and normal group were no significant difference. Conclusion; The concentration of BMMC intracellular Ca + rise is an important symbol of CAA disease situation. The Busui Shengxue particles can regulate Ca + release in BMMC, which may be one mechanism of the treatment of CAA. MDS,CAA,CITP, IDA' s Ca2+ concentrations have significant differences, which may be related to the disease' s degree of malignancy and mechanism.关键词
贫血/再生障碍性/慢性/Ca2+Key words
Anemia/ Aplastic/ Chronic/ Ca2 +分类
医药卫生引用本文复制引用
罗正凯,王金环,孙凤,雍延礼,孙伟正..补髓生血颗粒对慢性再障患者骨髓单个核细胞内Ca2+水平及不同血液疾病间差别的影响[J].中医药信息,2012,29(4):53-55,3.基金项目
国家自然科学基金项目(90709039) （90709039）
教育部博士学科点基金项目(200802280003)(20092327120001) （200802280003）
中国博士后基金项目(20100481034) （20100481034）
黑龙江省中医药管理局基金项目(ZHYO-W42) （ZHYO-W42）
黑龙江中医药大学基金项目(200901) （200901）
