安徽农业大学学报2012，Vol.39Issue(5)：735-738,4.
山羊α-乳清蛋白乳腺特异性表达载体的构建及核供体细胞的转染
Construction of mammary specific gland expression vector and transfection of nuclear donor cells
摘要
Abstract
The aim of this study was to construct a mammary gland-specific expression vector with a-lactalbumin gene and provide donor cells for mammary gland bioreactor. Total RNA was extracted from goat breast tissue and cDNA of a-lactalbumin gene was obtained by using RT-PCR technology. After the sequence of a-lactalbumin gene was identified by enzyme digestion, it was ligated into mammary gland-specific vector of pBC I -Neo. The recombinant vector sequence was identified by the enzyme digestion,and then transfected into goat fetal fibroblast by lipsome-mediated transfection. The transfected goat fetal fibroblast cells with mammary gland specific expression vector was screened by G418. As a result, the fraction of a-lactalbumin gene was properly placed into a mammary gland-specific expression vector, and recombint vector was steadily transfected into goat fetal fibroblast cells. Remaining the original morphology, the goat fetal fibroblast cells with transfected mammary gland-specific expression vector growed rapidly. In conclusion, mammary gland- specific expression vector was successfully constructed and steadily transfected goat fetal fibroblast cells for nuclear transfer were obtained.关键词
山羊/α-乳清蛋白基因/乳腺特异性表达载体/胎儿成纤维细胞/转染Key words
α-lactalbumin gene/ mammary special gland expression vector/ the goat fetal fibroblast cells/transfection分类
农业科技引用本文复制引用
任春环,曹鸿国,程箫,冯颖,张子军..山羊α-乳清蛋白乳腺特异性表达载体的构建及核供体细胞的转染[J].安徽农业大学学报,2012,39(5):735-738,4.基金项目
转基因生物新品种培育重大专项(2009ZX08006-007B). （2009ZX08006-007B）
