安徽农业大学学报2012，Vol.39Issue(5)：793-797,5.
冬虫夏草无性型菌丝体的原生质体制备条件研究
Conditions for protoplast preparation of Ophiocordyceps sinensis
摘要
Abstract
The mycelia of Hirsutella sinensis, the anamorph of O. sinensis, were cultured in liquid flasks and the different parameters for protoplast preparation of H. sinensis were investigated including the enzyme type and its concentration, stabilizer of osmotic pressure and its concentration, pH value of the enzyme and the enzymolysis time. The results showed that protoplasts were obtained with the highest yield of protoplast under the conditions as follows: digested by Yatalase enzyme of 4 mg/ml and Glucanex enzyme of 3 mg·mL-1, 1.0 mol·mL-1 KC1 as osmotic stabilizer, pH value of enzyme of 6.0 and enzymatic hydrolysis for 3 h.s关键词
冬虫夏草/中国被毛孢/原生质体/制备条件Key words
Ophiocordyceps sinensis/ Hirsutella sinensis/ protoplast/ preparation condition分类
农业科技引用本文复制引用
潘丹丹,肖岩岩,陈超,张磊,李春如..冬虫夏草无性型菌丝体的原生质体制备条件研究[J].安徽农业大学学报,2012,39(5):793-797,5.基金项目
国家高技术研究发展计划项目(863计划)(2007AA021506)和教育部留学回国人员科研启动基金(2009年)共同资助.. （863计划）
