安徽农业科学2012，Vol.40Issue(25)：12343-12344,12350,3.
薯蓣不同外植体愈伤组织培养的研究
Studies on Callus Tissue Culture for Different Explants of Dioscorea opposita
摘要
Abstract
The callus cultivating effects of different Dioscorea opposita explants were compared for establishing a high efficient plant regeneration system and providing a possible way to further use genetic transformation for variety improvement and new varieties of breeding. Results showed that the induction efficiency of different explants were obviously different on the same medium, which were shown as bulbil > shoot-tip > leaf and stem > tuber. The induction percentages of callus of the same explants were different on the medium supplied with different phyto-hormones concentration. The combination of MS + 1.0 mg/L 6-BA +0. 5 mg/L NAA was the best for callus induction from bulbil.关键词
薯蓣/外植体/愈伤组织/植物激素Key words
Dioscorea opposite/Explant/Callus/Phytohormone分类
农业科技引用本文复制引用
李朝炜,姚彬,王琳珊,黄亚辉..薯蓣不同外植体愈伤组织培养的研究[J].安徽农业科学,2012,40(25):12343-12344,12350,3.基金项目
河北省科技支撑计划项目(11220111D) （11220111D）
河北科技大学科学研究基金项目(XL200873). （XL200873）
