安徽农业科学2012，Vol.40Issue(25)：12376-12378,3.
水稻线粒体基因atp6超表达载体的构建及遗传转化
Construction and Genetic Transformation of Over-Expression Vector for atp6 Gene from Rice Mitochondria
摘要
Abstract
[Objective]This study aimed to investigate the biological effect of laser-induced mutation on fibrous roots of yellow skin onion.[Method]Wet seeds of two yellow skin onion cultivars were radiated by CO2 laser and He-Ne laser at three dosage levels separately. A randomized complete block design with three replications was adopted. The biological effect of laser-induced mutation on L,-generation fibrous roots of yellow skin onion was investigated with biostatistics and physiological and biochemical methods.[Result]Significant variations in the biological effects caused by various laser treatments were observed in the length, quantity, fresh weight and activity of onion adventitious roots. Specifically, the variation in adventitious root length induced by different types of laser reached 5% significance level; significant variation was observed in adventitious roots of different onion cultivars induced by laser, while the variation among each treatment did not reach 5% significance level; the variation in adventitious root quantity induced by different dosage levels of laser reached 5% significance level; laser radiation showed stimulating effect on root activity of onion.[Conclusion]This study provided reference for laser-induced breeding of yellow skin onion.关键词
水稻/atp6/遗传转化Key words
Rice/atp6/Genetic transformation分类
农业科技引用本文复制引用
熊磊,谭艳平,王春台,刘学群..水稻线粒体基因atp6超表达载体的构建及遗传转化[J].安徽农业科学,2012,40(25):12376-12378,3.基金项目
国家自然科学基金项目(31170226和31170296). （31170226和31170296）
