安徽农业科学2012，Vol.40Issue(25)：12393-12395,3.
利用ISSR标记解析紫花苜蓿·黄花苜蓿和胡卢巴属植物的亲缘关系
Analysis on Genetic Relationship among Medicago sativa, Medicago falcata and Trigonella foenum-graecum Using ISSR
摘要
Abstract
[Objective]To establish B. falcatum callus rapid propagation system.[Method]With the reference to the average growth of cells, the media, initial pH value, 6-BA, NAA and 2,4-D of B. fakatum callus were optimized by orthogonal optimal design method.[Result]The five factors had significant influence on the test results, especially N6, the initial pH of 5.6 was conducive to the growth of cells; of the three hormones, the largest cell fresh weight can be obtained when the combination of 6-BA (2 mg/L) and NAA (0. 5 mg/L) was added to the medium. The most advantageous medium for B. falcatum callus cell growth was N6 +2 mg/L 6-BA + 0. 5 mg/L NAA, pH 5.6. The cell growth of B. falcatum callus was inhibited by 2,4-D when it was added in medium.[Conclusion]The study results provide experimental data and theoretical support for further expanding the culture and establishing the suspension system of B. falcattan callus.关键词
紫花苜蓿/胡卢巴/黄花苜蓿/亲缘关系/ISSRKey words
Medicago sativa L./Medicago falcata L./Trigonella foenum-graecum L./Relationship/ISSR分类
农业科技引用本文复制引用
刘磊,王宗礼,李志勇,周国栋,师文贵,李鸿雁,蔡丽艳..利用ISSR标记解析紫花苜蓿·黄花苜蓿和胡卢巴属植物的亲缘关系[J].安徽农业科学,2012,40(25):12393-12395,3.基金项目
农业部牧草种质资源保护项目. （）
