安徽农业科学2012，Vol.40Issue(25)：12551-12552,12555,3.
人和哺乳动物精液的蛋白质组学研究进展
Research Advances in Human and Mammalian Semen Proteomic
摘要
Abstract
[Objective]The research aimed to optimize laboratory artificial rearing methods for Plutella xylostella L. and to select out effective insecticides against this pest species.[Method]Brassica chinensis seedlings were cultivated in the laboratory at (25 ±1 )℃ , 60 -75% RH, and a photoperiod of 14:10 ( L:D) h. The green blow molding papers were used to collect eggs, and Brassica oleracea seedlings were cultivated to breed Plutella xylostella L. until the insects were ready for use. Meanwhile, the susceptibility of 3rd instar larvae of Plutella xylostella L. to five types of insecticides was tested by leaf-dipping method. [Result]Plutella xylostella L. larvae were found well-grown and uniform in devel opmental stages with average pupa weight of 5.4 mg, pupa emergence percentage of 94.0 % and egg number of 135. 1 per female. The LC50, values of 3rd instar Plutella xylostella L. larvae against 200 g/L chlorantraniliprole SC, 20% flubendiamide WG, 150 g/L indoxacarb EC, 200 g/L tebufenozide SC and 1.8% abamectinEC, were 0.152, 0.223, 1.151, 93.340 and 0. 128 mg/L, respectively. [Conclusion]This rearing method is easy to be implemented and of great practicality, suitable for mass propagation of P. xylostella L. Abamectin, chlorantranil iprole and flubendiamide showed high insecticidal activities, so these three insecticides can be used alternately for the field prevention and con trol of Plutella xylostella L.关键词
蛋白质组学/精液/精子/精浆/研究进展Key words
Proteomic/Semen/Sperm/Seminal plasma/Research advances分类
农业科技引用本文复制引用
杨丽,付强,薛雯,张明..人和哺乳动物精液的蛋白质组学研究进展[J].安徽农业科学,2012,40(25):12551-12552,12555,3.基金项目
广西自然科学基金项目(0991047) （0991047）
广西科技厅基础条件平台建设项目(09-007-01) （09-007-01）
国家自然科学基金项目(31160421). （31160421）
