安徽农业科学2012，Vol.40Issue(25)：12619-12620,12697,3.
新技术、新工艺在园林工程中的应用
Application of New Technology and New Process in Landscape Engineering
卜卫东 1朱能武 1冯光澍1
作者信息
- 1. 广西生态职业技术学院,广西柳州545004
- 折叠
摘要
Abstract
[Objective]This study aimed to investigate the spatial-temporal dynamics of Bt toxic protein expression in insect-resistant transgen ic cotton and its degradation in soil.[Method]Btcryl Ac toxic protein expression in the roots, stems and leaves of transgenic cotton Cuoshen GK45 at different developmental stages and the annual average content of BtCryl Ac toxin protein in the topsoil, rhizosphere soil and later crop-growing area were explored and analyzed by using enzyme linked immuno sorbed assay ( ELJSA).[Result]The content of exogenous Bt Cryl Ac toxin protein decreased during the growth process of insect-resistant transgenic cotton, to be specific, the content of BtCryl Ac toxin protein in cotton stems and leaves decreased more slowly and always maintained a high level, while that in roots decreased rapidly and reached a minimum level to the later plant growth and development stage. BtCryl Ac toxin protein was detected in topsoil of both non-transgenic and transgenic cotton-growing areas, and the content of BtCryl Ac toxin protein increased in topsoil of later crop-growing area, which was very low in rhizosphere soil.[Conclusion]Determination of Btcryl Ac toxic protein provides scientific basis for the risk assessment of the cultivation of genetically modified crops and the safety evaluation of soil ecosystem.关键词
园林工程/新技术/新工艺/应用Key words
Landscape engineering/New technology/New process/Application分类
农业科技引用本文复制引用
卜卫东,朱能武,冯光澍..新技术、新工艺在园林工程中的应用[J].安徽农业科学,2012,40(25):12619-12620,12697,3.
