安徽农业科学2012，Vol.40Issue(25)：12666-12668,12670,4.
响应面优化酶法提取蛹虫草培养基多糖的研究
Optimization of Enzyme Extraction Conditions of Cordycepin Polysaccharide by Using Response Surface Methodology
安冬 1朱蓓薇2
作者信息
- 1. 大连工业大学食品学院,辽宁大连116034
- 2. 大连市农业科学研究院,辽宁大连116036
- 折叠
摘要
Abstract
[Objective]To study the lea extraction process, and determine the fluoride content in tea extracts l>y ion chrornatography under the optimized extraction conditions.[Method]The ion chromatography determination conditions were optimized, the lonpac AS9-HC anion sepa ration column and an eluant of 9 mmoL.Na2CO3, Here used for elution and determination.[Result]The linear range of the calibration curve for fluoride was 0.02 - 5. 00 (jLg/g with a detection limit of 1. 5 u,g/L The recoveries were 97. 62% - 102. 07% with RSD of 1. 01 % -1.63% in all teas. Content of fluoride in Tieguanyin and Pu-erh tea is higher than that in flower lea and black tea. Fluoride in green tea is (he minimum. Results obtained liy this method checked quite well with those obtained by standard method. In this work, content of fluoride in tea was all above the limited value recommended in GB, China.[Conclusion]Chromatography method can be well used in determination of fluor ide in tea extract, we suggest that people drink tea properly.关键词
培养基/蛹虫草多糖/酶法/响应面Key words
Cultivation leftover of Cordyceps milUaris/Cordycepin polysaccharide/Enzyme method/Response surface分类
农业科技引用本文复制引用
安冬,朱蓓薇..响应面优化酶法提取蛹虫草培养基多糖的研究[J].安徽农业科学,2012,40(25):12666-12668,12670,4.
