安徽农业科学2012，Vol.40Issue(26)：12754-12760,7.
牙鲆Toll样受体1基因全长cDNA的克隆及特征分析
Molecular Cloning and Expression of Toll-like Receptors 1 cDNA in Japanese Flounder,Paralichthys olivaceus
摘要
Abstract
[Objective] To clone the full-length gene of Toll-like receptors (TLRs) in Paralichthys olivaceus, and analyze their structural features and expression rules. [ Method ] The full-length cDNA sequence of Toll like receptor 1 ( TLR1) gene was identified from Paralichthys olivaceus head kidney by homologous cloning and rapid amplification cDNA ends (RACE). And then the bioinformatics and expression model of this gene were analyzed. [Result] The TLRA cDNA was 2 947 bp,an 2 418 bp open reading frame (ORF),encoding 805 amino acid (aa) residues,including signal peptide,6 leucine rich repeat(LRR) motifs, two transmembrane zones and one Toll/IL 1 receptor (TIR) domain. The molecular weight of the deduced protein was 91.15 kDa,and the isoelectric point was 6.49. The amino acid sequence of JfTLRl possessed 69% -35% identity with the TLRls of other vertebrates,further analysis showed that the TIR domain of JfTLRl shared 84% -62% identities with TIR domains in other vertebrates. JfTLRl protein firstly clustered with TLRls in Epinephelus coioides in the phylogenetic analysis. The transcription of JfTLRl was examined by real-time quantitative PCR,and its mRNA was mainly expressed in liver,heart and spleen. [Conclusion] The results lay a foundation for furthur studying the functions of TLR1 and exploiting the immune potentiator in Paralichthys olivaceus.关键词
牙鲆/Toll样受体1(TLR1)/qRT-PCRKey words
Paralichthys olivaceus/Toll-like receptor 1/qRT-PCR分类
农业科技引用本文复制引用
吴恋,孙金生,耿绪云,潘宝平,魏俊利,王雪惠,高虹..牙鲆Toll样受体1基因全长cDNA的克隆及特征分析[J].安徽农业科学,2012,40(26):12754-12760,7.基金项目
天津市自然科学基金(10JCYBJC09100)资助 （10JCYBJC09100）
天津师范大学博士基金(52XB1004)资助 （52XB1004）
天津师范大学市级重点实验室开放研究基金资助. （）
