安徽农业科学2012，Vol.40Issue(26)：12780-12781,2.
生物微核监测技术的改良方法研究
Study on Improved Methods of Biological Micronucleus Monitoring Technology
摘要
Abstract
[Objective] To study the improvement of biological micronucleus monitoring technology. [ Method] In view of the problems of present monitoring technology,an in-depth study was carried out on the selection of V.faba seeds,the length of root-tip length and the fixation and isolation in the process of microscopy producing. [Result] Chenghu No.3 was selected,and the most suitable growth length of root point was 1.05 -1. 10 cm and the improved fixed segregation liquid was absolute ethyl alcohol : glacial acetic acid : concentrated hydrochloric acid = 1:125:1 ;the fixation and isolation can be finished in one step,which only costs 4-5 min. [ Conclusion] The improved biological micronucleus monitoring technology is fast,stable and easy,thus can be widely promoted and applied.关键词
蚕豆/微核/监测技术/改良方法Key words
Vicia faba L. /Micronucleus/Monitoring technology/Improved methods分类
农业科技引用本文复制引用
王跃华,袁畅,杨小萍,王强,蔡锐,杨洪明..生物微核监测技术的改良方法研究[J].安徽农业科学,2012,40(26):12780-12781,2.基金项目
四川省教育厅项目(12ZB175). （12ZB175）
