安徽农业科学2012，Vol.40Issue(26)：12866-12867,12879,3.
酶法辅助提取南板蓝叶中靛玉红的工艺研究
Study on Enzyme-assisted Extraction of Indirubin from Baphicacanthus cusia (Nees) Bremek Leaves
摘要
Abstract
[Objective] To observe the key parameters of enzymatic extraction that impact extraction of indirubin from Baphicacanthus cusia (Nees) Bremek leaves. [Method] The content of indirubin was determined using HPLC. Effects of enzyme dosage, hydrolysis pH and hydrolysis time on extraction rate of indirubin were investigated with the aim of optimizing its extraction technology. [ Result] The optimal enzyme-assisted extraction conditions of indirubin from B. cusia leaves were the combination of enzyme dosage 25 U/g, hydrolysis pH 4. 8 and hydrolysis time 6 h. Under the above conditions, the Z value of indirubin was 7.02% . [Conclusion] Enzyme-assisted extraction method is suitable for extraction of indirubin from B. cusia leaves.关键词
酶法辅助提取/靛玉红(Indirubin)/南板蓝根(Baphicacanthus cusia(Nees)Bremek)Key words
Enzyme-assisted extraction/Indirubin/Baphicacanthus cusia (Nees) Bremek分类
农业科技引用本文复制引用
黄凤婷,马永良,林春颖,熊清平..酶法辅助提取南板蓝叶中靛玉红的工艺研究[J].安徽农业科学,2012,40(26):12866-12867,12879,3.基金项目
广州市卫生局中医科研课题资助项目(2009-A-50) （2009-A-50）
广东省中医药局建设中医药强省科研课题项目(2009303). （2009303）
