安徽农业科学Issue(27)：13283-13285,13420,4.
桃砧木GF677叶片愈伤组织诱导和保存
Studies on Callus Induction and Conservation from Leaves of Peach Rootstock GF677
摘要
Abstract
[Objective] To study the callus induction and conservation from leaves of peach rootstock GF677 (Prunus amygdalus×Prunus persica ). [ Method] Experiment was conducted with the leaves of peach rootstock GF677 in vitro for studying the effects of hormone dose, basic medium, AgNO3, dark culture time and carbon sources on callus induction and conservation. [ Result] The young leaves along with their petioles from 28-day-old in vitro grown shoots were used as explants, which were transferred to light culture after 21 d dark treatment. The result showed that the optimal medium for callus induction was LP + 1. 0 mg/L TDZ + 0.6 mg/L 6-BA +0.2 mg/L 2f 4-D + 0.5 mg/L Ag-NO3 + 20.0 g/L sorbitol +6.0 g/L agar. The optimal medium for callus conservation was LP + 0. 5 mg/L6-BA 4 0.5 mg/L 2, 4-D + 0. 2 g/L CH + 30.0 g/L sucrose + 6.0 g/L agar. The callus was in darkness all along and subcultured for every 21 d. [ Conclusion] The study laid a foundation for the genetic transformation and somaclonal variation of peach rootstock GP677.关键词
GF677/叶片/愈伤组织/诱导/保存Key words
GF677/ Leaf/ Callus/ Induction/ Conservation分类
农业科技引用本文复制引用
李果果,李国怀..桃砧木GF677叶片愈伤组织诱导和保存[J].安徽农业科学,2012,(27):13283-13285,13420,4.基金项目
国家桃产业技术体系专项(nycytx-31). （nycytx-31）
