安徽农业科学Issue(27)：13322-13323,2.
贵州不同产地苗药飞龙掌血中总生物碱和白屈菜红碱的含量测定
Determination of Total Alkaloid and Chelerythrine in Toddalia Asiatica from Different Places in Guizhou
摘要
Abstract
[Objective] To determine the contents of total alkaloid and chelerythrine in Toddalia asiatica from different places in Guizhou. [Method] Total alkaloid was determined by back-titrantion method, and chelerythrine was determined by HPLC method, using Diamonsil C18 column (250 mm×4.6 mm,5 μn) , and acetonitrile -0.1% phosphoric acid ( V/V, 28:72) as mobile phase, with the detection wavelength at 270 nm and flow rate of 1.0 ml/min. [ Result] The average recoveries of total alkaloid and chelerythrine were 97. 5% and 99 8% with RSD = 2.71% and 0.40% respectively. [Conclusion] This analysis method is accurate, sensitive and reliable to determine the contents of alkaloid in Toddalia asiatica.关键词
飞龙掌血/总生物碱/白屈菜红碱/高效液相色谱法Key words
Toddalia asiatica/ Total alkaloid/ Chelerythrine/ HPLC分类
农业科技引用本文复制引用
张艳焱,徐剑,张永萍..贵州不同产地苗药飞龙掌血中总生物碱和白屈菜红碱的含量测定[J].安徽农业科学,2012,(27):13322-13323,2.基金项目
贵州省科技厅基金资助项目(黔科合中药专字200407号). （黔科合中药专字200407号）
