安徽中医学院学报2012，Vol.31Issue(5)：65-68,4.DOI:10.3969/j.issn.1000-2219.2012.05.025
高效液相色谱法测定马兰中大黄酸、大黄素及大黄酚的含量
Determination of Rhein, Emodin, and Chrysophanol in Kalimeris Indica by High Performance Liquid Chromatography
摘要
Abstract
Objective To establish a method for determining the contents of rhein, emodin, and chrysophanol in Kalimeris indica by high performance liquid chromatography (HPLC). Methods HPLC was performed on Kromasil C18 column (250 mm×4. 6 mm, 5 μm) with a mobile phase of methanol-0. 1% phosphoric acid for gradient elution at a detection wavelength of 254 nm,a flow rate of 1 ml/min,and a column temperature of 30℃. Results The concentrations of rhein, emodin,and chrysophanol showed a good linear relationship with peak areas in 0. 253-2. 530 jug/ml (r =0.9996), 0.249-2.490 jug/ml (r = 0.9993), and 0.257-2.570 μg/ml (r=0. 9997), and the average recovery rates (n=6) were 99. 27% (RSD=1. 28%), 99.78% (RSD=1. 05%) , and 99. 92% (RSD=0. 83%) , respectively. Conclusion HPLC determination method can be used for measuring the contents of rhein, emodin, and chrysophanol in Kalimeris indica for its accuracy, reliability, and simplicity.关键词
马兰/大黄酸/大黄素/大黄酚/高效液相色谱法Key words
Kalimeris indica/ rhein/ emodin/ chrysophanol/ high performance liquid chromatography分类
医药卫生引用本文复制引用
张聪佴,钟文武,吴培云,刘劲松..高效液相色谱法测定马兰中大黄酸、大黄素及大黄酚的含量[J].安徽中医学院学报,2012,31(5):65-68,4.基金项目
安徽省自然科学基金项目(11040606W220) （11040606W220）
康缘中医药科技创新基金项目(KYCX 201003) （KYCX 201003）
