安徽中医学院学报2012，Vol.31Issue(5)：75-77,3.DOI:10.3969/j.issn.1000-2219.2012.05.028
超高效液相色谱法同时测定覆盆子中3种黄酮成分的含量
Simultaneous Determination of Three Flavonoids in Fructus Rubi by Ultra Performance Liquid Chromatography
摘要
Abstract
Objective To establish a method for determining the contents of three flavonoids in Fructus Rubi by ultra performance liquid chromatography (UPLC). Methods UPLC determination was carried out on a BEH C18 chromatographic column (2. 1 mm×100 mm, 1. 7 μm) with a mobile phase of acetoni-trile-0. 1% phosphoric acid (for gradient elution) at a flow rate of 0. 12 ml/min, a detection wavelength of 365 nm, and a column temperature of 20℃ to detect three flavonoids (linden glycosides, quercetin, and ru-toside). Results The contents of linden glycosides, quercetin, and rutoside had a good linear relationship with the peak areas within the ranges of 1. 31-13. 08 ng, 1. 92-19. 20 ng, and 49. 20-492. 00 ng, respectively. Their average recovery rates were 99. 7% , 99. 7% , and 100. 2% , respectively, and the relative standard deviations were 1. 03% , 0. 74% , and 1. 42% , respectively. The contents of three flavonoids in Fructus Rubi produced in different places and harvested at different times were significantly different. Conclusion The UPLC determination method can be used for the quality control of Fructus Rubi because of its simplicity and feasibility.关键词
覆盆子/黄酮/含量测定/超高效液相色谱法Key words
Fructus Rubi/ flavonoid/ determination/ ultra performance liquid chromatography分类
医药卫生引用本文复制引用
程丽珍,张玲,谢晓梅..超高效液相色谱法同时测定覆盆子中3种黄酮成分的含量[J].安徽中医学院学报,2012,31(5):75-77,3.基金项目
安徽省自然科学基金项目(090413101) （090413101）
安徽中医学院自然科学基金项目(2011zr0198) （2011zr0198）
