北京大学学报（医学版）2012，Vol.44Issue(4)：518-523,6.DOI:10.3969/j.issn.1671-167X.2012.04.006
StarD7与Wnt/β-catenin信号通路对Annexin 5刺激大鼠Leydig细胞睾酮分泌的影响
Effect of StarD7 and Wnt/β-catenin signal pathway on the testosterone secretion stimulated by Annexin 5 in rat Leydig cells
摘要
Abstract
Objective: This research aims to study the internal mechanism that promotes the testosterone synthesis by StarD7 and Wnt/β-catenin, and explores a new regulatory pathway of testosterone synthesis. Methods: After treated with 1 nmol/L Annexin 5 for 24 h, the culture media were collected for testosterone measurement by chemiluminescence assay. The expressions of StarD7 and β-catenin at mRNA and protein levels were detected by RT-PCR and western blot respectively. The cellular location of p-eatenin was identified by immunofluorescence. Results: Comparing with the control groups, under the treatment with Annexin 5 , the level of testosterone raised 176% [ (7. 83 ± 0. 32 ) vs. ( 21. 6 ± 1. 1 ) , P < 0. 05 ] , StarD7 mRNA in the experimental groups increased 55% [ ( 1. 12 ± 0. 08 ) vs. ( 1. 74 ± 0. 11 ) , P < 0. 05 ] , and p-catenin mRNA increased 48% [ (1. 15 ± 0. 08 ) vs. ( 1. 70 ± 0. 05 ) , P < 0. 05 ]. At the level of protein, the expression of StarD7 in the experimental groups increased 42% [ ( 1. 06 ±0. 09)vs. (1.51 ±0.07), P<0.05], and p-catenin increased 55%[(1.02 ±0.01) vs. (1.58 ±0.02), P< 0. 05 ]. Immunofluorescence identified that β-catenin was accumulation in the nuclear of the rat Leydig cells in the experiment groups cultured with Annexin 5. Conclusion: StarD7 and β-catenin have both increased significantly at the mRNA and protein levels under treatment with the Annexin 5, and β-catenin were accumulation in the nuclear of the rat Leydig cells. It suggests that StarD7 and β-catenin both regulate the effect of Annexin 5 in testosterone production of rat Leydig cells. This regulation may active the Wnt/β-catenin signal pathway, then increase the expression of the StarD7, eventually raise the progress of the testosterone secretion in rat Leydig cells.关键词
膜联蛋白5/睾丸间质细胞/睾酮/信号传导/磷蛋白类Key words
Annexin 5/ Leydig cells/ Testosterone/ Signal transduction/ Phosphoproteins分类
医药卫生引用本文复制引用
伏海燕,靖俊,伊男,余时超,恽时峰,梁元姣,姚兵..StarD7与Wnt/β-catenin信号通路对Annexin 5刺激大鼠Leydig细胞睾酮分泌的影响[J].北京大学学报（医学版）,2012,44(4):518-523,6.基金项目
国家自然科学基金(81070480)资助 （81070480）
