北京大学学报（医学版）2012，Vol.44Issue(5)：749-754,6.DOI:10.3969/j.issn.1671-167X.2012.05.016
高迁移率蛋白A2在浆液性卵巢癌中高表达及其与let-7家族microRNA的关系
Overexpression of high mobility group A2 and its correlation with microRNA let-7 family in serous ovarian cancers
摘要
Abstract
Objective:To examine the expression of high mobility group A2(HMGA2) , P53 and let-7 family microRNA, to investigate the correlation of HMGA2 and let-7 , and to compare the HMGA2 and P53 expressions in human serous ovarian cancer. Methods: Immunohistochemistry assay was used to examine the expressions of HMGA2 and P53 in 50 paraffin-embedded tissue specimens of human serous ovarian cancer and 4 normal fallopian tube tissues. HMGA2 mRNA and let-7 family microRNA were detected by real time fluorescent quantitative reverse transcription polymerase chain reaction in the corresponding frozen tissues. Results: HMGA2 and P53 were immuno-positive in 70% (35/50) and 78% (39/50) of the ovarian cancer tissues, respectively. HMGA2 was weakly expressed in the ciliated cells, but negative in the secretary cells of the fallopian tube. There was a tendency that the expression of HM-GA2 increased with higher pathological grade of the ovarian cancer, but no correlation was observed between the HMGA2 overexpression and clinical stages. HMGA2 mRNA was detected in all the ovarian cancer samples, and its expression level was higher than that of the normal fallopian tube tissues in 72% (36/50) of the ovarian cancer samples. The expression of HMGA2 mRNA was much higher in more malignant SKOV3. ipl cells than in its corresponding SKOV3 cells. All let-7 family members were detectable in all ovarian cancer samples, and their expression were inversely correlated with HMGA2 mRNA expression (r= -0. 305 ,P ＜0. 05). Conclusion: HMGA2 can be a biomarker complement to P53, and its high expression has an inclination of more malignancy. The downregulation of let-7 is, but not the only mechanism of HMGA2 overexpression in serous ovarian cancer.关键词
HMGA2蛋白/卵巢肿瘤/微RNAsKey words
HMGA2 protein/ Ovarian neoplasms/ MicroRNAs分类
医药卫生引用本文复制引用
杨静,张旗,董建强,昌晓红,何湘君..高迁移率蛋白A2在浆液性卵巢癌中高表达及其与let-7家族microRNA的关系[J].北京大学学报（医学版）,2012,44(5):749-754,6.基金项目
国家自然科学基金(30740047)资助 （30740047）
