北京中医药大学学报2012，Vol.35Issue(6)：426-429,后插4,5.
艾烟冷凝物对肺泡Ⅱ型上皮细胞A549活性及凋亡的影响
Influences of condensate of moxa smoke on viability and apoptosis of type Ⅱ alveolar epithelial cells (A549)
摘要
Abstract
Objective To observe the influences of condensate of moxa smoke (PM10, taken inhalable portion of moxa smoke) on viability and apoptosis of type Ⅱ alveolar epithelial cells ( A549 ). Methods A546 cells were cultured in vitro and then condensate of moxa smoke was added in different concentration. The influences of condensate of moxa smoke on the viability and apoptosis of A546 cells were observed by applying MTT assay and fluorescence microscope. Results The results of MTT assay showed that compared with control group, the survival rate of A546 cells changed with the changes of concentration and time showing an obvious time-dependence and a concentration-dependence. The condensate in the dose of 0. 12 mg/mL significantly improved the survival rate of A546 cells after acting on the cells for 12 hours (P =0. 005 <0. 01). The observation of fluorescence microscope showed that the apoptosis of A546 cells was induced by the condensate showing a concentration-dependence. Conclusion The viability of A546 cells decreases gradually as the increase of concentration and stimulating time of the condensate, which indicates that a certain concentration and a certain stimulating time of the condensate have some toxic effect on A546 cells. A certain concentration of the condensate can improve proliferation of A546 cells during a shorter stimulating time, which may be one of important factors of moxa smoke's effect. The apoptosis of A546 cells may be one of important factors of moxa smoked toxic effect.关键词
艾烟冷凝物/肺泡Ⅱ型上皮细胞/细胞活性/细胞凋亡Key words
condensate of moxa smoke/ typeⅡ alveolar epithelial cells/ cell viability/ apoptosis分类
医药卫生引用本文复制引用
胡海,赵百孝,邬继红,杨陟华,韩丽,蔡虹,朱茂祥..艾烟冷凝物对肺泡Ⅱ型上皮细胞A549活性及凋亡的影响[J].北京中医药大学学报,2012,35(6):426-429,后插4,5.基金项目
国家重点基础研究发展计划(973计划)资助项目(No.2009CB522906) （973计划）
