北京中医药大学学报2012，Vol.35Issue(7)：461-465,469,6.
黄芪注射液对复苏后多器官功能障碍综合征中血管内皮细胞损伤的影响
Influence of Huangqi Injection on vascular endothelial injury in multiple organ dysfunction syndrome after anabiosis
摘要
Abstract
Objective To investigate the influence of Huangqi Injection on vascular endothelial injury in multiple organ dysfunction syndrome ( MODS) after anabiosis with different resuscitation fluid through establishing the model of MODS in rats with two hits. Methods SD rats (n = 32) were randomly divided into control group, RL group (resuscitated with Lactated Ringers Solution) , HTH group (resuscitated with 7.5% hypertonic saline and 6% hydroxyethyl starch mixture) , and HTH group was divided again into HTH1 group (without Huangqi Injection after anabiosis) and HTH2 group (with Huangqi Injection after anabiosis). The animal model of MODS after anabiosis was copied in RL group and HTH group. HTH2 group was given intraperitoneal Huangqi Injection and HTH1 group and RL group was given 0. 9% sodium chloride solution. After 24 hours the blood samples and lung tissue samples were collected for detecting the number of serum circulating endothelial cells ( CEC ) , and content of nitric oxide (NO ) , endothelin-1 (ET-1) , lung tissue tumor necrosis factor-a (TNF-oc) and intercellular adhesion molecule-1 (ICAM-1), interleukin-6 (IL-6), vascular cell adhesion molecule-1 (VCAM-1) mRNA.and pathological changes of lung tissue were examined with light microscope. Results The number of CEC and content of NO and ET-1 were significantly lower in HTH2 group than those in RL group and HTH1 group (all P < 0.05) . The content of TNF-α, ICAM-1, IL-6 and VCAM-1 were significantly lower in HTH2 group than those in HTH1 group (all P <0. 05) . Conclusion Huangqi Injection has obvious protective effect on vascular endothelial of MODS after anabiosis with different resuscitation fluid.关键词
黄芪/多脏器功能障碍综合征/血管内皮细胞/大鼠Key words
Huangqi Injection/ multiple organ dysfunction syndrome/ vascular endothelial cells/ rats分类
医药卫生引用本文复制引用
陈骏,段绍斌,杨东鹰,居来提,史志涛,吾买尔,刘郁,张增峰..黄芪注射液对复苏后多器官功能障碍综合征中血管内皮细胞损伤的影响[J].北京中医药大学学报,2012,35(7):461-465,469,6.基金项目
新疆维吾尔自治区卫生厅中医民族医药青年科技人才专项基金资助项目(No.2007ZMY07) （No.2007ZMY07）
