北京中医药大学学报2012，Vol.35Issue(7)：470-474,5.
四逆汤组成药物不同配伍对Lewis肺癌小鼠血清血管内皮生长因子表达的影响
Influences of Sini Tang and different combinations of its medicinals on expression of serum vascular endothelial growth factor in mice with Lewis lung cancer
摘要
Abstract
Objective To study the influences and anti-relapse and anti-metastasis effects of Sini Tang and its different combinations of medicinals on expression of serum vascular endothelial growth factor ( VEGF) in mice with Lewis lung cancer. Methods The effects of different combinations of Fuzi (Radix Aconiti Laleralis praeparata) , Ganjiang ( Rhizoma Zingiberis ) and prepared Gancao ( prepared Radix Glycyrrhizae) in Sini Tang were observed by using orthogonal design method. C57BL/6 mice (n =102) were divided into 17 groups according to orthogonal array ( each n - 6 ). On the second day of establishment of Lewis lung cancer model, all groups were intragastrically given medicinals (60 mg/kg) every day. On the 15th day, all mice were sacrificed by cervical dislocation. The tumor inhibitor)' rate was calculated after weighing tumor tissue. The pathological changes of tumor tissue were observed, number of metastatic nodules of pulmonary serface was counted, and changes of serum VEGF were observed by using double-antibody sandwich ELISA. Results Sini Tang had influence in varying degrees on tumor weigh. In group 4,6,7 and 10, the tumor weight and tumor weight index were significantly lower than those in group 1 (control group, P<0.05) and in group 17 (complete Sini Tang group, P < 0.05) , and were most significant in group 10 (P <0. 01) . The tumor inhibitory rate was the highest in group 4,6,7 and 10. The number of metastatic nodules had statistical significance in the comparison between group 5 , 7 , 11 and 16 and group 1 ( P < 0.05) , and less in group 7,11 and 16 than that in group 1. The expression of serum VEGF was significantly different in the comparison between group2,3,6,7,10,12,12,13,14,16 and 17 and group 1 (p<0.01). Conclusion In Sini Tang, Fuzi, Ganjiang and prepared Gancao have the inhibitory effect on the proliferation of Lewis lung cancer, and the effect is the most significant when the combination of these three medicinals in the proportion of 3:1:6.关键词
四逆汤/肺癌/血管内皮生长因子/小鼠Key words
Sini Tang/ lung cancer/ vascular endothelial growth factor/ mice分类
医药卫生引用本文复制引用
高静东,陈嘉璐,李湧健,张蕾,刘敏,张晓迪,黄帅,金文伟,龙军..四逆汤组成药物不同配伍对Lewis肺癌小鼠血清血管内皮生长因子表达的影响[J].北京中医药大学学报,2012,35(7):470-474,5.基金项目
苏州吴中医药中西医结合科研基金资助项目(No.SZD09152) （No.SZD09152）
