草业科学2012，Vol.29Issue(9)：1384-1389,6.
尖叶胡枝子种子贮藏蛋白分析
Analysis on seed storage protein of Lespedeza hedysaroides
摘要
Abstract
Seed storage proteins of three Lespedeza hedysaroides were analyzed by SDS-PAGE technique. Results indicated that SDS-PAGE of salt-soluble proteins and seed storage proteins were practical and reliable methods for determining relationships among three L. hedysaroides. Salt-soluble protein and seed storage protein in the common-type and deep green-type L. hedysaroides were significantly different from those in the tall-type. The protein molecular weight ranged from 10. 375 KDa to 155. 53 KDa. 6 bands were shared and 13 bands were polymorphism （68.42%） of total 19 salt-soluble protein bands. 19 bands were shared and 9 bands were polymorphism （32.14%） of total 28 seed storage protein bands. Clustering showed that three L. hedysaroides were divided into two groups. The common-type and the deep greentype were classified as the first group and the tall-type one was second group. Therefore, SDS-PAGE technology is an important method to study intraspecies variation among Lespedeza species.关键词
尖叶胡枝子/种子贮藏蛋白/SDs_PAGEKey words
Lespedeza hedysaroides/seed storage protein/SDS-PAGE分类
农业科技引用本文复制引用
王清郦,孙启忠,赵淑芬,郭艳艳,周国栋..尖叶胡枝子种子贮藏蛋白分析[J].草业科学,2012,29(9):1384-1389,6.基金项目
现代农业产业技术体系（CARS-35）建设专项资金 （）
