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苦豆子种质资源遗传多样性的RAPD分析

阳翠 张晓岗 杨玉蓉 刘萍

草业科学2012,Vol.29Issue(11):1692-1697,6.
草业科学2012,Vol.29Issue(11):1692-1697,6.

苦豆子种质资源遗传多样性的RAPD分析

Genetic diversity of Sophora alopecuroides germplasm resources based on RAPD analysis

阳翠 1张晓岗 1杨玉蓉 1刘萍1

作者信息

  • 1. 宁夏大学农学院,宁夏银川750021
  • 折叠

摘要

Abstract

Genetic diversity of Sophora alopecuroides germplasm resources was analyzed at DNA level by Random Amplified Polymorphic DNA (RAPD). A total of 314 DNA hands from 22 populations in different habitats were amplified with 31 reliable RAPD primers, of which 285 DNA bands (90. 76%) were polymorphic. The Nei's gene diversity (H) and Shannon information index (I) were 0. 301 5 and 0. 453 1, respectively. The genetic distances ranged from 0. 114-0. 709. The results of RAPD unweighted pair- group method with arithmetic means (UPGMA) cluster and the principal component analysis (PCA) dem- onstrated that 22 S. alopecuroides populations were clustered into 6 groups, which means that the genetic differentiations exist among populations.

关键词

苦豆子/RAPD/遗传分化/亲缘关系/聚类分析

Key words

Sophora alopecuroides/RAPD/genetic differentiation/genetic relationship/cluster analysis

分类

农业科技

引用本文复制引用

阳翠,张晓岗,杨玉蓉,刘萍..苦豆子种质资源遗传多样性的RAPD分析[J].草业科学,2012,29(11):1692-1697,6.

基金项目

国家自然科学基金 ()

宁夏大学自然科学基金 ()

草业科学

OA北大核心CSCDCSTPCD

1001-0629

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