草业科学2012,Vol.29Issue(11):1692-1697,6.
苦豆子种质资源遗传多样性的RAPD分析
Genetic diversity of Sophora alopecuroides germplasm resources based on RAPD analysis
摘要
Abstract
Genetic diversity of Sophora alopecuroides germplasm resources was analyzed at DNA level by Random Amplified Polymorphic DNA (RAPD). A total of 314 DNA hands from 22 populations in different habitats were amplified with 31 reliable RAPD primers, of which 285 DNA bands (90. 76%) were polymorphic. The Nei's gene diversity (H) and Shannon information index (I) were 0. 301 5 and 0. 453 1, respectively. The genetic distances ranged from 0. 114-0. 709. The results of RAPD unweighted pair- group method with arithmetic means (UPGMA) cluster and the principal component analysis (PCA) dem- onstrated that 22 S. alopecuroides populations were clustered into 6 groups, which means that the genetic differentiations exist among populations.关键词
苦豆子/RAPD/遗传分化/亲缘关系/聚类分析Key words
Sophora alopecuroides/RAPD/genetic differentiation/genetic relationship/cluster analysis分类
农业科技引用本文复制引用
阳翠,张晓岗,杨玉蓉,刘萍..苦豆子种质资源遗传多样性的RAPD分析[J].草业科学,2012,29(11):1692-1697,6.基金项目
国家自然科学基金 ()
宁夏大学自然科学基金 ()