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30份野牛草种质的遗传多样性

成凯凯 魏小兰 杜鹃 张蕴薇

草业科学2012,Vol.29Issue(11):1698-1705,8.
草业科学2012,Vol.29Issue(11):1698-1705,8.

30份野牛草种质的遗传多样性

A study on genetic diversity of Buchloe dactyloides with different geographical origin

成凯凯 1魏小兰 1杜鹃 1张蕴薇1

作者信息

  • 1. 中国农业大学草地研究所,北京100193
  • 折叠

摘要

Abstract

30 Buchloe dactyloides collected from different geographical origins were put into this research. The basic turf characters, including leafblade length, leafblade width, height, diameter, number of tillers, number of stolon, length of stolon inter node, length of stolon, leaf color, extent of leaf turning yellow were measured and the genetic diversity was analyzed using the ISSR technique. The following results were obtained: 1) The difference of leaf color among these germplasm was significant (P~0.05) , and the difference of leafblade length, leafblade width, height, diameter, length of stolon, number of stolon was very significant (P~0.01). 2) Seven primers produced 108 polymorphic bands, with an average of 15.6 bands per primer. The percentage of polymorphic bands in average was 82.6%. 3) The Nei's genetic simi- larity coefficient (GS) of the tested accessions ranged from 0. 546 to 0.88, which suggested that there was rich genetic diversity among the germplasm of B. dactyloides. 4)These accessions were clustered into five groups. Moreover, there was the positive correlation between the geographical distance and the genetic distance.

关键词

野牛草/坪用性状/ISSR/遗传多样性

Key words

Buchloe dactyloides/turf characters/ISSR/genetic diversity

分类

农业科技

引用本文复制引用

成凯凯,魏小兰,杜鹃,张蕴薇..30份野牛草种质的遗传多样性[J].草业科学,2012,29(11):1698-1705,8.

基金项目

科技支撑 ()

973课题 ()

863课题 ()

国家现代牧草产业技术体系 ()

草业科学

OA北大核心CSCDCSTPCD

1001-0629

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