草业学报2012，Vol.21Issue(4)：98-104,7.
柳枝稷人工穗芽高效再生体系的建立
Establishment of a highly efficient regeneration system of switchgrass artificial spike buds
摘要
Abstract
With inflorescence of three genotypes of Xiji No. 1, Xiji No. 2, Xiji No. 3 as materials, using single factor design consider factors including genotype, panicle length, phytohormone, the length of spike buds in the initial cutting period and in mass cultivating period, the carbon source, pH, spike buds cutting way and root induction medium, to optimize the regeneration system of switchgrass (Panicum virgatum) artificial spike buds. The optimal regeneration system was: genotype Xiji No. 1, panicle length 1. 5 cm, 3. 0 mg/L 6-BA+0. 1 mg/L 2,4-D as phytohormone, the length of spike buds in the initial cutting period and in mass cultivating period was 1. 5 cm, maltose as carbon source, pH 5. 8, vertical spike buds cutting, and 1/2 MS as root induction medium. The proliferation efficiency of switchgrass artificial spike buds might reach up to 4 167. 00%.关键词
柳枝稷/幼穗/人工穗芽/再生体系Key words
switchgrass (Panicum virgatum) /panicle/artificial spike buds/regeneration分类
农业科技引用本文复制引用
柴乖强,徐开杰,王勇峰,孙风丽,宋立立,刘曙东,奚亚军..柳枝稷人工穗芽高效再生体系的建立[J].草业学报,2012,21(4):98-104,7.基金项目
国家自然科学基金(30871571,31171607),国家948计划项目(2011-G1-19)和西北农林科技大学基本科研业务费专项资金(CX200903)资助. （30871571,31171607）
