草业学报2012，Vol.21Issue(4)：159-167,9.
紫花苜蓿WRKY转录因子基因的克隆与亚细胞定位
Cloning and subcellular localization of a WRKY transcription factor gene of Medicago sativa
摘要
Abstract
The WRKY transcription factors play important roles in plant growth and development. A cDNA sequence of Medicago sativa WRKY transcription factor gene was isolated by electronic cloning and RT -PCR (reverse transcription polymerase chain reaction) , and was named MsWRKY56. The cDNA sequence was 1 267 bp and contained a maximum open reading frame of 951 bp coding for 317 amino acids. Subcellular localization analysis of a construct with the transient expression vector of the MsWRKY56 gene showed that the MsWRKY56 protein was localized in the nucleus and was thus consistent with the general character of subcellular localization of transcription factors. This research forms a basis to further study the structure and function of the gene encoded protein.关键词
紫花苜蓿/WRKY转录因子基因/瞬时表达载体/亚细胞定位Key words
Medicago sativa /WRKY transcription factor gene/transient expression vector/subcellular localization分类
农业科技引用本文复制引用
陈婷婷,杨青川,丁旺,康俊梅,张铁军,张新全..紫花苜蓿WRKY转录因子基因的克隆与亚细胞定位[J].草业学报,2012,21(4):159-167,9.基金项目
现代农业产业技术体系建设专项资金(编号:CARS-35)资助. （编号:CARS-35）
