草业学报2012，Vol.21Issue(4)：168-174,7.
冷诱导转录因子 AtCBF1转化紫花苜蓿的研究
A study on Agrobacterium tumefaciens-mediated transformation of Medicago sativa with the AtCBF1 gene
摘要
Abstract
The cold resistance effect of the cold-induced transcription activator CBFl (C-repeat-binding-factor) gene on Medicago sativa was studied by amplifying an AtCBFl gene and cloning it by polymerase chain reaction (PCR) from the genomic DNA of Arabidopsis thaliana. The length of the DNA cloned fragment was 642 bp. Compared with the CBFl sequence in GenBank, the sequence homology rate was 99. 84%. On the basis of the CBFl gene, the plant expression vector pBI121-CBFl was constructed with the AtCBFl gene and M. sativa was transformed using Agrobacterium tumefaciens. The transformed plants were selected with kanamycin. An electrophoretic band of 650 bp was obtained by PCR and RT-PCR (reverse transcription polymerase chain reaction). The AtCBFl gene was expressed in M. sativa. This research provides a good basis for breeding new M. sativa varieties with cold resistance.关键词
紫花苜蓿/CBF1基因/遗传转化/抗寒性Key words
Medicago sativa /CBFl gene/genetic transformation/cold resistance分类
农业科技引用本文复制引用
徐春波,王勇,赵海霞,米福贵..冷诱导转录因子 AtCBF1转化紫花苜蓿的研究[J].草业学报,2012,21(4):168-174,7.基金项目
内蒙古自然科学基金项目(2011MS0402)和中央级公益性科研院所基本科研业务费专项资金(中国农业科学院草原研究所)项目(1610332011003)资助. （2011MS0402）
