大豆科学2012，Vol.31Issue(4)：593-597,5.
栽培大豆和野生大豆及其回交后代苗期耐盐性分析
Analysis of Seedlings Salt Tolerance of Backcross Hybrids of Glycine max and Glycine soja
摘要
Abstract
In this study,the hybrid strain( F5 )3060 selected for salt tolerance generation by generation from the cross combination ( Glycine max, Lee68 cultivar x Glycine soja, N23227 population) was used as the male parent, the Lee68 was used as the female parent or recurrent parent, the backcross combinalion ( Lee68 × 3060, named as C) was configured, and several single hybrid strains( BC1 F2 ) were obtained. Using these soybean materials, their salt tolerance was evaluated with parameters including the salt tolerance coefficient,relative growth rate and dry matter accumulation. On that basis,the salt tolerance mechanism of one fine strain was investigated with parameters, such as the relative electrolytic leakage, thiobarbituric acid reactive substances (TBARS) content, Pn and the maximum photochemical efficiency of photosystem Ⅱ ( Fv/Fm). The results showed that, under salt stress,the salt tolerance coefficient,relative growth rate and dry matter accumulation in C3 and C10 hybrid strains were obviously higher than those in their female parent Lee68, and almost be equal to or more than those in the male parent 3060 strain, especially the C10 strain displayed the most. When compared with their two parents, the increase of the relative e-lectrolytic leakage and TBARS content in roots and leaves,and the decrease of Pn and Fv/Fm in leaves of C10 seedlings under salt stress were distinctly lower. Moreover,the relative electrolytic leakage and TBARS content in roots and leaves of C10 seedlings under salt stress were not significantly different compared to its control. So,the C10 hybrid strain could be considered as one excellent salt tolerant soybean germplasms for future study.关键词
栽培大豆/野生大豆/回交后代/耐盐性Key words
Glycine max/ Glycine soja/ Backcross hybrids/ Salt tolerance分类
农业科技引用本文复制引用
李发院,田芳,张晓可,於丙军..栽培大豆和野生大豆及其回交后代苗期耐盐性分析[J].大豆科学,2012,31(4):593-597,5.基金项目
国家自然科学基金项目(30871462) （30871462）
国家转基因生物新品种培育重大专项(2009ZX08004-008B). （2009ZX08004-008B）
