中德临床肿瘤学杂志（英文版）2012，Vol.11Issue(10)：592-597,6.DOI:10.1007/s10330-012-0920-6
BRMS1基因对人胃癌细胞增殖和转移作用的体内研究
Effect of breast-cancer metastasis suppressor 1 (BRMS1) on growth and metastasis of human gastric cancer cells in vivo
摘要
Abstract
Objective: The aim of the study was to investigate inhibitory effects of breast-cancer metastasis suppressor 1 protein (BRMS1) on primary tumor growth and metastasis of human gastric cancer (GC) cells in nude mice. Methods: We compared the expression of BRMS1 in the primary gastric tumor and metastatic gastric tumor by immunohistochemistry.Expression of BRMS1 also was detected in the GC cells by RT-PCR and Western blot. Three groups of cultured human GC passage in the subcutis of nude mice, were transplanted orthotopically into stomach walls of nude mice to establish a nude mouse model of human gastric carcinoma. Their primary tumor growth and metastasis were then observed. Results: The expression of BRMS1 was markedly stronger in the primary gastric tumor compared with metastatic gastric tumor. We also detected BRMS1 gene and protein in the gastric cancer cell lines. Numbers of metastasis tumors significantly differed among mice infected with transfected cells, with negative controls and with blank controls (4.38 ± 0.60, 7.75 ± 0.59, and 7.63 ± 0.65,respectively; P < 0.05). However, there were no significant differences in the size of orthotopic tumors among mice infected with transfected, negative control and blank control cells [(12.02 ± 0.70), (12.71 ± 0.63) and (12.89 ± 0.71) mm, respectively;P > 0.05]. Conclusion: BRMS1 suppresses metastasis of GC cells, but does not inhibit growth of gastric tumors.关键词
gastric carcinoma (GC)/breast-cancer metastasis suppressor 1 (BRMS1)/metastasisKey words
gastric carcinoma (GC)/breast-cancer metastasis suppressor 1 (BRMS1)/metastasis引用本文复制引用
Zhigang Jie,Xiaoping Chen,Zhengrong Li,Yi Liu,Yinliang Li,Xing Zou,Huaicheng Yang,Deyu Xiang..BRMS1基因对人胃癌细胞增殖和转移作用的体内研究[J].中德临床肿瘤学杂志（英文版）,2012,11(10):592-597,6.基金项目
Supported by a grant from the Jiangxi Provincial Natural Science Foundation of China (No.0640063). （No.0640063）
