大连理工大学学报2012，Vol.52Issue(4)：476-480,5.
百合无症病毒衣壳蛋白基因克隆和蛋白分析
LSV coat protein genes cloning and protein analyses
摘要
Abstract
A pair of primers are designed and synthesized based on the oligosaccharides nucleotlde sequences of coat protein （CP） genes of lily symptomless virus （LSV） reported, then, a fragment of 876 bp of LSV CP gene sequence is amplified by reverse transcription polymerase chain reaction （RT- PCR） from the total RNA infected lily leaves. The function and the secondary structure of LSV CP are predicted by the bioinformatics software system after analyzing the sequence. The results show that the gene is 876 bp in length coding 291 amino acids. This isolation has a homology of 93.4~- 99.0~~ at nucleotide and 84. 8~-99. 5~ at amino acid levels respectively compared with those published sequences of LSV according to GeneBank. The functional study prediction shows that the grand average of hydropathicity is --0.432. The protein has one coiled-coil domain, several phosphorylation sites and a conserved structure domain of Carlaviruses CP. The secondary structure is mainly composed of a-helix and random coil-based structures.关键词
百合无症病毒/衣壳蛋白/序列分析/功能预测Key words
lily symptomless virus/coat protein/sequence analysis/functional prediction分类
农业科技引用本文复制引用
徐品三,刘纪文,高晓蓉,尹雅蕾..百合无症病毒衣壳蛋白基因克隆和蛋白分析[J].大连理工大学学报,2012,52(4):476-480,5.基金项目
国家自然科学基金资助项目（30771760,31070621）. （）
