大连工业大学学报2012，Vol.31Issue(5)：322-325,4.
抗大肠杆菌荧光抗体的制备及其特性
Preparation and characteristics of anti-E. coli fluorescent antibody
孙庆元 1王建军 1刘建文 1郇晓娜 1朱友双1
作者信息
- 1. 大连工业大学生物工程学院,辽宁大连 116034
- 折叠
摘要
Abstract
E. coli in vitro with incomplete Freund s adjuvant was emulsificated and injected into New Zealand white rabbits. Using two-step methods of precipitation, IgG of anti-protein of E. coli cell surface was successfully extracted and purified from the rabbits in 15 d after injection. The rate of the IgG cross-linked with FITC reached to 2. 7. It was not found significant effect on the specificity of the antibody to E. coli surface protein. When the FITC-antibody of anti-E. coli was injected into mice, fluorescence was observed only in the large intestine rather than other organs or tissues such as the liver, heart, and kidney. The results showed that E. coli existed only in the mouse large intestine and the FITC-antibody of anti-E. coli could be used to examine and determine E. coli qualitatively and quantitatively.关键词
大肠杆菌/免疫/异硫氰酸荧光素/荧光抗体Key words
E. coli/ immune/ FITC/ fluorescence-antibody分类
生物科学引用本文复制引用
孙庆元,王建军,刘建文,郇晓娜,朱友双..抗大肠杆菌荧光抗体的制备及其特性[J].大连工业大学学报,2012,31(5):322-325,4.
