动物学研究2012，Vol.33Issue(4)：389-394,6.DOI:10.3724/SP.J.1141.2012.04389
文昌鱼tropomyosin基因的克隆、进化分析及其胚胎发育与成体中的表达模式
Phylogenetic analysis and expression patterns of tropomyosin in amphioxus
摘要
Abstract
In amphioxus, we found a mesoderm related gene, tropomyosin, which encodes a protein comprising 284 amino acid residues, sharing high identities with other known Tropomyosin proteins both in vertebrates and invertebrates. Phylogenetically, amphioxus Tropomyosin fell outside the invertebrate clade and was at the base of the vertebrate protein family clade, indicating that it may represent an independent branch. From the early neurula to the larva stage, whole-mount in situ hybridization and histological sections found transcripts of amphioxus tropomyosin gene. Weak tropomyosin expression was first detected in the wall of the archenteron at about 10 hours-post-fertilization neurula stage, while intense expression was revealed in the differentiating presumptive notochord and the muscle. Transcripts of tropomyosin were then expressed in the formed notochord and somites. Gene expression seemed to continue in these developing organs throughout the neurular stages and remained till 72-hours, during the early larval stages. In situ study still showed tropomyosin was also expressed in the neural tube, hepatic diverticulum, notochord and the spaces between myotomes in adult amphioxus. Our results indicated that tropomyosin may play an important role in both embryonic development and adult life.关键词
文昌鱼/进化分析/胚胎发育/表达图式/TropomyosinKey words
Amphioxus/ Phylogenetic analysis/ Embryogenesis/ Expression pattern/ Tropomyosin分类
生物科学引用本文复制引用
李忻怡,林浴霜,张红卫..文昌鱼tropomyosin基因的克隆、进化分析及其胚胎发育与成体中的表达模式[J].动物学研究,2012,33(4):389-394,6.基金项目
国家自然科学基金资助项目(30700434) （30700434）
