动物营养学报2012，Vol.24Issue(8)：1438-1446,9.DOI:10.3969/j.issn.1006-267x.2012.08.007
谷氨酰胺对断奶仔猪生长性能、营养物质表观消化率、空肠碱性磷酸酶活性及与肠道健康相关因子基因表达的影响
Effects of Glutamine on Growth Performance, Apparent Digestibility of Nutrients, Jejunal Alkaline Phosphatase Activity and Expression of Genes Related to Intestinal Health in Weaner Piglets
摘要
Abstract
The aim of the present paper was to provide a profile of the effects of Gin on growth performance, apparent digestibility of nutrients, jejunal alkaline phosphatase activity and expression of genes related to intestinal health in weaner piglets. One hundred and twenty-eight 21-day-old piglets (Duroc x Landrace x Yorkshire) were randomly assigned into control group and 1. 00% Gin group according to their body weight and sex, and each group contained 4 replicates and 16 piglets per replicate. The fresh feces samples were collected in each replicate on days 10 and 30 post-weaning, and one piglet was selected for jejunum sample collection based on its weight near to the average weight of the corresponding group. The experiment lasted for 30 days. Results showed that average daily gain, the apparent digestibility of dry matter (DM) , crude protein (CP) , and apparent metabolizable energy (AME) in the Gin group were increased by 19. 05% , 9. 06% , 4.77% and 7.02% (P <0. 05) compared with the control group on day 10 post-weaning, and the apparent digestibility of most amino acids in the Gin group were also increased (P <0. 05) ; the apparent digestibility of DM, CP, and AME were increased by 5. 90% , 2.80% and 6.43% (P <0.05) on day 30 post-weaning. Jejunal brush border membrane-bound alkaline phosphatase activity in the Gin group was increased by 30. 09% (P <0. 05) and 5.98% (P>0.05) on days 10 and 30 post-weaning, respectively. Peroxisome proliferator-activated receptor gamma (PPAR-y) and pyruvate kinase (PK) mRNA levels were decreased by 10. 00% (P >0. 05) and 30. 00% (P >0. 05) on day 10 post-weaning, and by 41. 88% and 44. 40% (P < 0. 05) on day 30 post-weaning. Gin supplementation increased the mammalian target of rapamycin ( mTOR) mRNA levels by 22. 00% (P >0. 05) and 22. 38% (P <0. 05) on days 10 and 30 post-weaning. These data indicate that Gin increases jejunal alkaline phosphatase activity and expression of genes related to intestinal health, which improves growth performance and apparent digestibility of nutrients in weaner piglets.关键词
谷氨酰胺/断奶仔猪/表观消化率/碱性磷酸酶/基因表达Key words
glutamine/ weaner piglets/ apparent digestibility/ alkaline phosphatase/ gene expression分类
农业科技引用本文复制引用
肖英平,洪奇华,刘秀婷,刘锐钢,赵许可,陈安国,杨彩梅..谷氨酰胺对断奶仔猪生长性能、营养物质表观消化率、空肠碱性磷酸酶活性及与肠道健康相关因子基因表达的影响[J].动物营养学报,2012,24(8):1438-1446,9.基金项目
"十一五"国家科技支撑计划重点项目(2006BAD14B01) （2006BAD14B01）
宁波市重大科技攻关计划项目(2008C10023) （2008C10023）
