动物营养学报2012，Vol.24Issue(8)：1512-1519,8.DOI:10.3969/j.issn.1006-267x.2012.08.015
中华蜜蜂铜锌超氧化物歧化酶基因的克隆、序列分析及表达特征
Cloning, Sequence Analysis and Expression Profile of Copper/Zinc Superoxide Dismutase Gene of Apis cerana cerana
摘要
Abstract
This experiment was conducted to clone the cDNA and study the expression profile of copper/zinc superoxide dismutase (SOD1) gene of Apis cerana cerana. The SOD1 gene of Apis cerana cerana was cloned by nested PCR. The expression level of SOD1 gene in different tissues (brain, thorax and abdomen of adults) and at different developmental stages (3- and 6-day-old larva instars, 1- and 4-day-old pupas and 1- and 7-day-old adults) of Apis cerana cerana was detected using quantitative real-time PCR (qRT-PCR). The results showed that the full length of cDNA of SOD1 gene of Apis cerana cerana (GenBank accession number is JN700517) was 631 bp, encoding 152 amino acids, and the predicted molecular weight and isoelectric point were 15.65 ku and 6. 21, respectively. The deduced amino acid sequence of SOD1 of Apis cerana cerana showed the highest similarity (99% ) with that of Apis mellifera ligustica, and had 67% ~85% similarity with other insects (such as Drosophila melanogaster, Anopheles gambiae, Spodoptera exigua, Bombyx mori, Bom-bus ignitus and so on). The expression level of SOD\ gene was significantly different at different developmental stages of Apis cerana cerana, and the peak appeared in 6-day-old larva instar, while the lowest value appeared in 4-day-old pupa. The expression level in the brain and abdomen was significantly higher than that in the thorax (P <0. 05). The cDNA of SOD1 gene of Apis cerana cerana is successfully cloned in this study, and the full length is 631 bp, encoding 152 amino acids. The expression profile at different developmental stages and in different tissues is different.关键词
中华蜜蜂/铜锌超氧物歧化酶/克隆/序列分析/表达特性Key words
Apis cerana cerana/ copper/zinc superoxide dismutase/ cloning/ sequence analysis/ expression profile分类
农业科技引用本文复制引用
刘俊峰,刘亭亭,王欢,王子龙,颜伟玉,曾志将,吴小波..中华蜜蜂铜锌超氧化物歧化酶基因的克隆、序列分析及表达特征[J].动物营养学报,2012,24(8):1512-1519,8.基金项目
国家公益性行业(农业)科研专项"畜禽饲料营养价值与饲养标准研究与应用"(200903006) （农业）
