南方医科大学学报2012，Vol.32Issue(9)：1250-1254,5.DOI:10.3969/j.issn.1673-4254.2012.09.007
PKC介导凝血酶诱导人肺成纤维细胞单核细胞趋化蛋白-1的表达
Protein kinase C mediates thrombin-induced monocyte chemoattractant protein-1 release from human lung fibroblasts
摘要
Abstract
Objective To investigate the role of protein kinase C (PKC) in thrombin-induced monocyte chemoattractant protein-1 (MCP-1) release by human lung fibroblasts (HLF-1). Methods Cultured human lung fibroblasts HLF-1 were incubated with different concentrations of PKC inhibitors before by thrombin stimulation. MCF-1 protein levels in She supemaiants were assessed using ELBA, and MCP-1 mRNA levels in the cell lysate were measured by quantitative real-time PCR. Results The broad spectrum PKC inhibitors bisindolylmaleimide I and RO-31-8220 obviously inhibited thrombin-induced MCP-1 mRNA and protein expressions in HLF-1 cells, whereas Ca2+-dependent PKC inhibitor Go 6976 had no such effects. Conclusion Ca2+-independent PKC mediates thrombin-induced MCP-1 release in cultured HLF-1 cells.关键词
凝血酶/蛋白激酶C抑制剂/单核细胞趋化蛋白-1/人肺成纤维细胞Key words
thrombin/ protein kinase C inhibitor/ monocyte chemoattractant protein-1/ human lung fibloblasts分类
医药卫生引用本文复制引用
袁超,许铭炎,陈耿臻,傅玉才,邓小玲..PKC介导凝血酶诱导人肺成纤维细胞单核细胞趋化蛋白-1的表达[J].南方医科大学学报,2012,32(9):1250-1254,5.基金项目
国家自然科学基金(30900661) （30900661）
教育部留学回国人员科研启动基金[教外司(2012)1567号)] （2012）
汕头大学医学院科研基金 （）
