南方医科大学学报2012，Vol.32Issue(9)：1270-1273,4.DOI:10.3969/j.issn.1673-4254.2012.09.011
白纹伊蚊C6/36细胞登革Ⅱ型病毒结合分子的筛选
Screening of dengue Ⅱ virus-binding molecules from Aedes albopictus C6/36 cells
摘要
Abstract
Objective To screen the molecules binding dengue II virus expressed in Aedes albopictus C6/36 cells and characterize their biological functions. Methods Aedes albopictus C6/36 cells were infected with dengue II virus, and the virus were collected and purified. The total and membrane proteins of C6/36 cells were extracted and analyzed using 12% SDS-polyacrylamide gel (PAGE). After electophoresis, the proteins were transferred to a nitrocellulose membrane, and virus overlay protein-binding assay (VOPBA) was carried out using an anti-dengue virus 1-4 monoclonal antibody. Results Two specific bands of 67 000 and 30 000 occurred after VOPBA of the proteins from the cells incubated with the virus, while the negative control group did not show these specific bands. Conclusion Two putative dengue virus receptor molecules of 67 000 and 30000 have been obtained from C6/36 cells using VOPBA, and their functional identification is in progress .关键词
白纹伊蚊C6/36细胞/登革Ⅱ型病毒/结合分子/筛选Key words
Aedes albopictus C6/36 cells/ dengue II virus/ virus binding molecules/ screening分类
医药卫生引用本文复制引用
郑学礼,雷子庆,潘京..白纹伊蚊C6/36细胞登革Ⅱ型病毒结合分子的筛选[J].南方医科大学学报,2012,32(9):1270-1273,4.基金项目
国家自然科学基金(30872198,30972566) （30872198,30972566）
