南方医科大学学报2012，Vol.32Issue(9)：1307-1311,5.DOI:10.3969/j.issn.1673-4254.2012.09.020
呼吸道白念珠菌分离株生物膜表型差异的机制
Mechanism of phenotype difference in biofilm formation by Candida albicans isolated from the respiratory tract
摘要
Abstract
Objective To explore the molecular mechanism of the differences in biofilm formation abilities of Candida albicans isolated from the respiratory tract. Methods The biofilms formed by Candida albicans isolates from the respiratory tract and the standard strain ATCC90028 were examined for bacterial proliferation using XTT reduction assay. The mRNA expression of CPH1, EFG1, ALS3 and HWP1 in the isolates were measured with fluorescent quantitative RT-PCR. Results XTT reduction assay demonstrated a strong ability of biofilm formation in 8 clinical isolates, and a relatively low biofilm formation ability in 7 clinical isolates and ATCC90028 strain. The strong and weak biofilm formers showed significant differences in ALS3 and HWP1 mRNA expressions (P<0.05) but not in EFG1 or CPH1 mRNA expressions (P>0.05). Conclusion The clinical isolates from the respiratory tract have different biofilm formation abilities under regulation by genes other than the transcription factors CPH1 and EFG1.关键词
白念珠菌/生物膜/转录因子Key words
Candida albicans/ biofilm/ transcription factors分类
医药卫生引用本文复制引用
阳隽,张天托,朱家馨..呼吸道白念珠菌分离株生物膜表型差异的机制[J].南方医科大学学报,2012,32(9):1307-1311,5.基金项目
广州市医药卫生科技一般引导项目(2008-YB-161) （2008-YB-161）
