福建农林大学学报（自然科学版）2012，Vol.41Issue(5)：518-522,5.
丁香假单胞菌极毛蛋白hrpA基因的克隆与表达
Cloning and expression of hrpA gene of Pseudomonas syringae
摘要
Abstract
HrpA gene was obtained from Pseudomonas syringae pv. Tomato DC3000 by PCR and the protein expression system of Escherichia coli BL21 (DE3) for hrpA was constructed with vector pET32a( + ). SDS-PAGE analysis showed that the transformant with recombinant expression plasmid pET32a ( + ) - hrpA produced a fusion protein of TrxA-HrpA with corresponding molecular weight of 28 ku. Furthermore, HrpA recombination protein was purified through Ni2 + -NTA and its concentration was measured to be 91.8 g·L-1.关键词
丁香假单胞菌/hrpA基因/融合蛋白/表达/纯化Key words
P. Syringae/ hrpA gene/ fusion protein/ expression/ purification分类
生物科学引用本文复制引用
袁军,刘海荣,庄振宏,汪世华..丁香假单胞菌极毛蛋白hrpA基因的克隆与表达[J].福建农林大学学报（自然科学版）,2012,41(5):518-522,5.基金项目
国家自然科学基金资助项目(30771400) （30771400）
福建省自然科学基金资助项目(2009J06008,2011J05049). （2009J06008,2011J05049）
