福建农林大学学报（自然科学版）2012，Vol.41Issue(5)：529-533,5.
红车轴草提取物对脂多糖应激断奶仔猪生长性能和炎性介质的影响
Effect of red clover extract on growth performance and inflammatory parameters of weaned pigs with lipopolysaccharide challenging
摘要
Abstract
To study the effects of red clover extract (REC) on growth performance and inflammatory parameters of weaned pigs with lipopolysaccharide (LPS) challenging. Thirty healthy Duroc x Landrace x Yorkshire crossbred weaned piglets with body weight of (6.86 ±0.14) kg were randomly allocated to 5 groups. Every group included 6 replicates and 1 piglet in per replicate. The five treatments groups include: basic ration groups (no LPS control groups), basic ration groups + LPS control groups ( LPS control groups) , basic ration groups + LPS control groups +0.05% RCE groups (0.05% RCE groups) , basic ration groups + LPS control groups +0.10% RCE groups (0.10% RCE groups), basic ration groups + LPS control groups +0.20% RCE groups (0.20% RCE groups). On the 21st day after weanling, experiment piglets in groups LPS, 0.05% RCE, 0.10% RCE and 0.20% RCE were injected intraperitoneally with 80 μg · kg-1 LPS every other day (on day 7, 7 and 14 of experiment) , whereas pigs in groups 1 were injected with an equivalent amount of sterile saline. The results showed: (1) Compared with the blank control groups and LPS groups, from day 1 to 7 (before IPS challenge) , the average daily gain of piglets in the 0.10% RCE groups increased significantly (P <0.05) and average feed-gain ratio decreased significantly (P<0.05). While there was no significant difference among 0.05% RCE groups, 0.20% RCE groups and control groups on average daily gain of piglets and feed/gain ratio (P>0.05). From day 7 to 21 ( with LPS challenge twice) , the average daily gain of piglets and average daily feed intake in IPS groups decreased significantly (P <0.05) and average feed-gain ratio increased significantly (P <0.05) compared with the blank control groups. While the average daily gain of piglets and average daily feed intake in 0.05% REC groups, 0.1% REC groups, 0.20% RCE groups decreased, but not significantly (P >0.05). (2) After injected intraperitoneally with LPS every other day (on day 7 and 14 of experiment) , the serumal IgG levels of piglets in the IPS groups were significantly lower than those in control groups (P < 0.05) , while the se-rumal cytokine IL-6 and tumor necrosis factor TNF-α were both increased significantly ( P < 0.05). There was no significant difference between 0.05% RCE groups and IPS groups on serumal IgG levels, serumal cytokine IL-6 and tumor necrosis factor TNF-α( P > 0.05 ). The serumal IgG levels of piglets in the 0.1 % REC groups and 0.2% REC groups increased significantly ( P < 0.05 ) , but the serumal cytokine IL-6 and tumor necrosis factor TNF-α both decreased significantly ( P > 0.05 ). The dietary supplementation with 0.1% REC could significantly relieve the weaned piglets challenged by LPS. Compared with the LPS groups, the serumal IgG levels increased by 38.65% (P<0.05), serumal cytokine IL-6 decreased by 32.81% (P<0.05) and TNF-α both decreased by 24.68% ( P < 0.05). The result indicated that the dietary supplementation with 0.1% REC was best, it can alleviate the growth depression and inflammatory cytokine secretion change of weaned piglets challenged by IPS to some degree.关键词
红车轴草提取物/脂多糖/断奶仔猪/生长性能/炎性介质Key words
red clover extract/ lipopolysaccharide/ weaned pigs/ growth performance/ inflammatory parameters分类
农业科技引用本文复制引用
何玉琴,杨小燕,林标声,邱龙新..红车轴草提取物对脂多糖应激断奶仔猪生长性能和炎性介质的影响[J].福建农林大学学报（自然科学版）,2012,41(5):529-533,5.基金项目
福建省科技计划重点项目(2010N0023) （2010N0023）
龙岩市科技计划项目(2012LY44、2011LY18). （2012LY44、2011LY18）
