福建农业学报2012，Vol.27Issue(7)：711-716,6.
福建野生葡萄松散型愈伤组织的诱导及其继代保持
Friable Callus Induction of Fujian Wild Grapes (Vitis Amurensis Rupr.) and Its Subculture and Maintenance
摘要
Abstract
The plantlets of Fujian wild grapes (Vitis amurensis Rupr. ) were established by using its stems with node as explants. And its callus induction, subculture and long-term maintenance were carried out by using the stems and leaves of these plantiets. The results showed that, with the illumination of lights, the effect of medium MS+BA 2. 0 mg · L-1 + NAA 0. 2 mg · L-1 + Sucrose 30 g · L-1 revealed the best on inducing of calluses for a rate of 100%. The medium MS+2,4-D 1.0 mg · L-1 + Sucrose 30 g · L-1 was the best for the callus subculture multiplication to obtain the friable calluses in good growth. For a long-term maintenance, the calluses were sub-cultured for several generations with the medium MS+2,4-D 1. 0 mg · L-1 + Sucrose 30 g · L-1 and then followed with the sub-culture of medium MS+2.4-D 1. 0 mg · L-1+ KT 0. 5 mg · L-1 +AgNO3 5. 0 mg · L-1+ Sucrose 30 g · L-1 for one generation. Continually, the calluses were alternately sub-cultured with the two mediums and could grow vigorously with the illumination of lights.关键词
葡萄/外植体/愈伤组织/诱导/继代保持Key words
grape (Vitis vini fera L. )/ explant/ callus/ induction/ subculture and maintenance分类
农业科技引用本文复制引用
范丽华,赖呈纯,谢鸿根,余亚白..福建野生葡萄松散型愈伤组织的诱导及其继代保持[J].福建农业学报,2012,27(7):711-716,6.基金项目
福建省自然科学基金项目(2012J01102) （2012J01102）
福建省科技计划项目——省属公益类科研院所基本科研专项(2011R1017-2) （2011R1017-2）
福建省农业科学院博士科研启动基金项目(2010BS-3) （2010BS-3）
