分析测试学报2012，Vol.31Issue(8)：940-944,5.DOI:10.3969/j.issn.1004-4957.2012.08.009
层层自组装纳米金与乙酰胆碱酯酶电化学生物传感器检测有机磷农药
Detection of Organophosphate Pesticides with Electrochemical Biosensors Based on Layer-by-layer Self-assembly Acetylcholinesterase and Gold Nanoparticles
摘要
Abstract
An amperometric biosensor for the detection of organophosphorus pesticide was developed based on layer-by-layer self-assembly both acetylcholinesterase (AChE) and gold nanoparticles (AuNPs) on glassy carbon electrodes(GCE) in the presence of poly ( diallyldimethylammonium chloride) (PD-DA). The electrochemical properties of the biosensor were investigated by electrochemical impedance spectroscopy and differential pulse voltammetry. The proposed biosensor exhibited a high affinity to the substrate of AChE and produced a detectable and fast response owing to its excellent electron transfer rate and satisfactory biocompatibility of AuNPs. Compared with that at the unmodified electrode , the peak current of thiocholine at the proposed biosensor obviously increased. Under the optimum conditions, the inhibition rate of methyl parathion to the immobilized AChE was proportional to the logarithm of concentration of methyl parathion over the range of 4. 6 ×10 -5 - 5. 3 × 10 -3 mol/L with a detection limit of 7. 6 × 10 -6 mol/L. The proposed biosensor was successfully applied in the determination of methyl parathion in vegetable sample, which means that the proposed biosensor provides a new promising tool for the pesticide analysis.关键词
生物传感器/层层自组装/乙酰胆碱酯酶/金纳米粒子/有机磷Key words
biosensor/ self-assembly/ acetylcholinesterase/ gold nanoparticles/ organophosphorus分类
化学化工引用本文复制引用
薛瑞,康天放,鲁理平..层层自组装纳米金与乙酰胆碱酯酶电化学生物传感器检测有机磷农药[J].分析测试学报,2012,31(8):940-944,5.基金项目
北京市自然科学基金资助项目(8102009) （8102009）
北京市教委科技发展基金资助项目(KZ201110005006) （KZ201110005006）
国家自然科学基金资助项目(20247002,21005005) （20247002,21005005）
