高等学校化学学报2012，Vol.33Issue(8)：1692-1697,6.DOI:10.3969/j.issn.0251-0790.2012.08.011
基于目标物诱导置换及纳米金催化的新型荧光技术检测腺苷
Novel Fluorescent Technique for Detecting Adenosine Based on DNA Strand Displacement Induced by Target-aptamer Complex and Metal Deposition Catalyzed by Gold Nanoparticles
摘要
Abstract
Using adenosine as model analyte, a novel homogeneous fluorescent method with high sensitivity and specificity was proposed. It involves two kinds of DNA probe conjugated nanoparticles-aptamer modified magnetic nanoparticles and capture DNA probe derivatized gold nanoparticles (GNPs) , which were connected together via hybridization. The conformational transition of aptamer induced by adenosine-aptamer complex contributes to the displacement of probe conjugated GNP, which further catalyzes the reduction of Cu2+ by ascorbic acid, causing the deposition of Cu atom on the surface of GNP. The fluorescence quenching of calcein was restored as the consumption of Cu2+. The experimental results demonstrated that the dynamic range for adenosine detection was from 100 pmol/L to 10 nmol/L, with a detection limit of 80 pmol/L. The proposed method shows high selectivity for the specific recognition of adenosine by its aptamer.关键词
纳米金/催化沉积/磁性纳米颗粒/核酸适体/荧光猝灭抑制Key words
Gold nanoparticles/ Catalytic deposition/ Magnetic nanoparticles/ Aptamer/ Inhibition of fluorescence quenching分类
化学化工引用本文复制引用
刘雪平,欧阳湘元,吴会旺,沈国励,俞汝勤..基于目标物诱导置换及纳米金催化的新型荧光技术检测腺苷[J].高等学校化学学报,2012,33(8):1692-1697,6.基金项目
国家自然科学基金(批准号:21025521, 21035001)和国家“九七三”计划项目(批准号:2011CB911000)资助. （批准号:21025521, 21035001）
