国际医药卫生导报2012，Vol.18Issue(20)：2943-2947,5.DOI:10.3760/cma.j.issn.1007-1245.2012.20.001
肾血管性高血压犬中肾动脉狭窄的超声检测及价值
The value and ultrasonic detection of renal artery stenosis in renovascular hypertensive canine
摘要
Abstract
Objective To investigate the ultrasonic detection method ant influence on model establishment of renal artery stenosis in renovascular hypertensive canine.Methods Twelve beagles in this experiment were assigned to stenosis rate ＞ 80％,70％ ～ 80％ and sham-operated group according to the bilateral renal artery diameter stenosis,and induced renovascular hypertension by 2 kidney 2 clip (2k2c) method.One week after operation,the diameter on the stenosis site and the distal segment of renal artery and the corresponding peak systolic velocity (PSV) in constricted beagles were detected by color ultrasound, and compared with sham-operated beagles to calculate the stenosis rate.The serum urea nitrogen (BUN) and creatinine (Cr) on the preoperative 1st day and the postoperative 2nd day,the 4th,the 8th and the 12th month were dynamically measured.One year after operation,the histology findings of kidney and carotid artery were compared between constricted and sham animals.Results In the preliminary stage of the experiment,2 beagles consecutively died of acute renal failure within 4 postoperative days,so this method was given up.All 5 beagles with the bilateral renal artery constriction of between 70％ and 80％ developed hypertension, 7 days after operation, the stenosis rates of left and right renal artery were respectively revealed by color ultrasound as (73.9 ± 4.2)％ and (73.2 ± 4.4)％,which were consistent with the ratios of the PSV on the stenosis site of left and right constricted renal artery and the mean PSV of left and right sham renal artery.One year after operation,histology findings identified transparent degeneration in part renal tissues and obvious carotid plaque formation in constricted beagles,and the BUN and Cr didn' t exhibit significant changes either.None of 5 sham beagles became hypertensive,and kidney and carotid artery histology didn' t show observable abnormal features 1 year after operation.Conclusions An appropriate stenosis degree of renal srtery is the key tenique of 2k2c method in the establishment of renovascular hypertensive canine medel; color ultrasound can reveal the accurate stenosis degree of renal artery in renovascular hypertensive beagles,which may make a guidance for the experiment.关键词
肾血管性高血压/肾动脉狭窄/超声Key words
Renovascular hypertension/Renal artery stenosis/Ultrasound引用本文复制引用
田作军,由天辉,廖海星,王晓明,陈长兵,沈粤春,陈惠芬,曾玲,汤庆,陆亚琴..肾血管性高血压犬中肾动脉狭窄的超声检测及价值[J].国际医药卫生导报,2012,18(20):2943-2947,5.基金项目
广东省自然科学基金项目(7301663 （）
10151009504000007) （）
广东省社会发展领域科技计划项目(粤科函社字[2010]1096号) （粤科函社字[2010]1096号）
广东省医学科研基金(A2010224) （A2010224）
广东省大学生创新项目(1057310039) （1057310039）
